Encyclopedia Of Experiments

Immune Systems and Components

This collection features experimental procedures focused on the isolation, culture, differentiation, and functional analysis of various immune cells. These techniques are designed to investigate different aspects of the immune system and its cellular components. They involve the manipulation and study of immune cells derived from both human and mouse models.

Encyclopedia Of Experiments

Immune Systems and Components

This collection features experimental procedures focused on the isolation, culture, differentiation, and functional analysis of various immune cells. These techniques are designed to investigate different aspects of the immune system and its cellular components. They involve the manipulation and study of immune cells derived from both human and mouse models.

Sections

Cell Isolation and Culture

Cell Differentiation Techniques

Assays and Functional Profiling

Cell Isolation and Culture

Cell Differentiation Techniques

Assays and Functional Profiling

Cell Isolation and Culture

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection

Isolation and Culture of Mouse Kupffer Cells

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation

Isolation of Lymphocytes from a Mouse Peyer's Patch

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats

Isolation and Purification of B Cells from Human Peripheral Blood

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules

Selective Isolation of CD4⁺ Vδ1⁺ γδ T Cells from Human Peripheral Blood Mononuclear Cells

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity

Magnetic Isolation of Microglia from Mouse Pup Brains

Harvesting an Inguinal Draining Lymph Node from a Murine Model

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

Cell Differentiation Techniques

In Vitro Culture and Differentiation of Primary Monocytes into Macrophages

Differentiation of Human-Induced Pluripotent Stem Cells into Macrophages

Differentiation and Polarization of Monocyte-Derived Cells into Macrophage-Like Cells

In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors

Generation of Immature, Mature, and Tolerogenic Dendritic Cells from Monocytes

In Vitro Mouse Bone Marrow Stromal Cell Differentiation into Adipocytes

An In Vitro Method for the Differentiation of Megakaryocytes and Platelet Formation

Three-Dimensional Thymic Culture System to Generate iPSC-Derived Thymic Emigrants

Purification and Activation of Platelets from Murine Whole Blood

An In Vitro Technique to Generate Human Neutrophil Extracellular Traps

An In Vitro Technique to Differentiate GM-CSF Producing T Helper Cells from Naïve T Cells

Assays and Functional Profiling

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

Flow Cytometry-Based Cell Sorting for Different Circulatory B Cell Populations

Functional Assessment of Antibody-Secreting B Cells Using ELISpot Assay

Identifying Regulatory T Cell Subsets from Murine Pancreatic Draining Lymph Nodes

Immunological Synapse Formation Between Helper T Cells and Antigen-Presenting Cells

CD Marker Recognition of Human Cytokine-Induced Killer Cells by Flow Cytometry

Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs

Characterization of Macrophage Extracellular Traps in Mouse Lung Tissue Samples

Preparing an Antigen-Adjuvant Emulsion to Induce Autoimmune Encephalomyelitis

Intravital Visualization of Gamma Delta Intraepithelial Lymphocytes

Isolation of Microparticles Derived from Apoptotic T Lymphocytes In Vitro

Nanoparticle-Based Enrichment and Expansion of Antigen-Specific CD8+ T Cells

Label-Free Neutrophil Separation from Tracheal Secretion Using Spiral Microfluidics Channel

Labeling Murine T Helper Lymphocytes using Radiolabeled Monoclonal Antibodies