Encyclopedia Of Experiments

Antibody-Based Technologies

This collection features techniques for antibody production and purification, assays to assess their functionality and specificity, and approaches for their application in imaging and detection, or analysis of various biological samples or conditions. This resource supports researchers in leveraging antibodies for scientific discovery and therapeutic advancements in biomedical research.

Encyclopedia Of Experiments

Antibody-Based Technologies

This collection features techniques for antibody production and purification, assays to assess their functionality and specificity, and approaches for their application in imaging and detection, or analysis of various biological samples or conditions. This resource supports researchers in leveraging antibodies for scientific discovery and therapeutic advancements in biomedical research.

Sections

Production and Purification

Characterization and Functional Analysis

Antibody-Based Imaging and Detection Methods

Production and Purification

Characterization and Functional Analysis

Antibody-Based Imaging and Detection Methods

Production and Purification

Production and Purification of Recombinant Antibodies from Mammalian Cells

Generating Recombinant Monoclonal Antibodies From Mammalian Cell Cultures

A Hybridoma Technology for Producing Monoclonal Antibodies against a Metallopeptidase

A Method to Produce Recombinant Antibodies Against Metallopeptidase

A Technique to Generate Monoclonal Antibodies from Antigen-Specific B Cells

A Technique to Purify Monoclonal Antibodies Produced by Chinese Hamster Ovary Cells

Antibody Production from a Batch Overgrowth Culture of Transfected Mammalian Cells

An Affinity Chromatography Technique for the Purification of Monoclonal Antibodies

Characterization and Functional Analysis

Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies

An Assay to Detect Serum Anti-aquaporin-4 Immunoglobulin G

Generation of Influenza Virus Escape Variants against Neutralizing Antibodies

An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies

A Cell-Based Assay to Assess the Tau Protein Uptake by Microglia

An Assay to Detect Autoantibodies in Human Serum using a Hippocampal Neuronal Culture

An Assay to Measure the Neutralizing Antibody Titer Specific for Respiratory Syncytial Virus

Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay

A Paper-Based Immunoassay for the Detection of Immunoglobulin G

An In Vitro Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilm

An Assay to Measure Influenza Neuraminidase Inhibition Antibody Titers

A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients

Detecting Circulating Anti-Glycan Antibodies with a Printed Glycan Array

A Peptide Microarray Technology for Specificity Profiling of Antibodies

Immunolocalization of Arabinogalactan Proteins and Pectins in Plant Tissue

Using a Sandwich ELISA to Determine the Immunogenic Glycoprotein Content in a Vaccine

A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E

Detecting Abnormal Prion Proteins in Brain Tissue Using Immunohistochemistry

Evaluating the Intracellular Distribution of Virus-Derived Peptide-Modified Antibody Conjugates

An In Vitro Technique to Study Glutamate Receptor Trafficking in Hippocampal Neurons

An In Vitro Assay to Measure Antibody-Dependent Cellular Phagocytosis by Phagocytes

An Assay to Quantify the Binding of Human Norovirus VLPs to Intestinal Bacteria

A Capillary Electrophoresis Immunoassay for Protein Biomarker Detection

Measuring Immunoglobulin G Levels Against a Test Vaccine in a Mouse Serum

An Assay to Measure the Accumulation of a Fluorescent Antibiotic Probe in Bacterial Cells

Antibody-Based Imaging and Detection Methods

In Vivo Fluorescence Imaging to Localize Antibodies in a Mouse Tumor Xenograft Model

Convection-Enhanced Delivery of Antibodies into a Mouse Brain

An Ultrasound-Assisted Delivery of Antibodies in a Mouse Model

An In Vivo PET Imaging Technique to Detect Tumors in a Murine Model Using Radiolabeled Antibodies

An Immunofluorescence-Based Method for Visualizing Tuft Cells in Jejunum Cryosections

An In Vivo Technique to Track Bispecific Antibody-Induced T Cell Trafficking to Tumors

A Double-Labeling Immunofluorescence Technique to Visualize Host and Pathogen Proteins in an Infected Cell

An Immunofluorescence Staining Technique to Detect Adult Hippocampal Neurogenesis

An Immunolabeling Technique to Visualize the Subcellular Localization of Proteins

A Whole-Mount Organ Staining Technique for Three-Dimensional Imaging of the Kidney

An Immunofluorescence Assay to Detect Alpha-Synuclein Aggregates in a Skin Biopsy Section

Using Fluorescence-Mediated Tomography to Assess Murine Intestinal Inflammation

An Antibody Uptake Assay for Imaging Notch/DeltaD Signaling in Zebrafish Radial Glia Progenitors

Immunocapture and Flow Cytometry of Extracellular Vesicles for Antigenic Characterization

A Multiplex Bead-Based Immunoassay to Quantify Multiple Cytokine Targets

Flow Cytometry-Based Analysis of Dendritic Cell Activation Using Immune Complexes

A Technique to Detect Cyanobacterial Strains Using an Antibody Microarray Chip

Immunostaining of Cells Encapsulated in a 3D Hydrogel System

Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus

Target Peptide Detection and Measurement Using a Capacitive Immunoprobe Biosensor

Assessing T-cell Receptor-Induced Calcium Influx Using a Calcium Indicator Dye

An In Vivo Assay to Detect Mitophagy in Transgenic Nematodes

A Magnetic Nanoparticle-Based Immunoassay Using a Microfluidic Device

An Immunofluorescence-Based Method to Quantify Replication Stress in Cancer Cells

A Snap Chip Technology for a Cross-Reactivity-Free Multiplexed Sandwich Immunoassay

A Multiplex Immunostaining Method Using Primary Antibodies from the Same Host Species

Multiplexed Immunofluorescence Imaging to Analyze Immunotherapy-Resistant T Cell Subpopulations

An Immunofluorescence Technique for Viral Protein Localization in Infected Cells

An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs

Assessing Caspase-Mediated Cleavage of Viral and Host Proteins in Virus-Infected Cells