Encyclopedia Of Experiments

Immunotherapy

This collection showcases experimental protocols in immunotherapy, highlighting innovative approaches like CAR T-cell production, siRNA treatments, and novel vaccines. Using techniques like CRISPR-Cas9 genetic editing and viral transduction, these protocols explore immune system dynamics to develop treatments for a range of complex diseases. These protocols are key to advancing both the theoretical and practical aspects of immunotherapy.

Encyclopedia Of Experiments

Immunotherapy

This collection showcases experimental protocols in immunotherapy, highlighting innovative approaches like CAR T-cell production, siRNA treatments, and novel vaccines. Using techniques like CRISPR-Cas9 genetic editing and viral transduction, these protocols explore immune system dynamics to develop treatments for a range of complex diseases. These protocols are key to advancing both the theoretical and practical aspects of immunotherapy.

Sections

Cellular Immunotherapy

Model Development and Disease Study

Immunogenics and Vaccine Development

Cellular Immunotherapy

Model Development and Disease Study

Immunogenics and Vaccine Development

Cellular Immunotherapy

Isolating Invariant Natural Killer T Cells from a Mouse Model

An Ex Vivo Technique to Manufacture Chimeric Antigen Receptor T Cells

Enhancing Viral Transduction in Natural Killer Cells Using a Cationic Polymer

Transfecting Primary Macrophages with Modified mRNA

Near-Infrared Photoimmunotherapy for Targeted Cell Elimination in Mixed 3D Cultures

siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells

Suppressing Hepatitis B Virus Replication Using Viral Antigen-Specific T Cells

An Ex Vivo Technique to Generate Tumor-Specific Chimeric Antigen Receptor T Cells

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System

A CRISPR-Cas9 Technique for Gene Editing in T Cells

A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9

An In Vitro Assay to Assess CAR T Cell Cytotoxicity against Tumor Spheroids

A Three-Dimensional Co-culture Model of Infiltrating T Cells in Tumor Spheroids

An In Vitro Assay to Evaluate the Anti-Tumor Activity of Tumor-Specific CAR T Cells

An In Vitro Assay for Evaluating Natural Killer Cell Cytotoxicity against Cancer Cells

A Technique for the Generation of Antigen-Specific CAR T Cells

Generating Plasma Membrane Vesicles from Bone Marrow Stem Cells

Generating Anti-Angiogenic Tumor-Associated Neutrophils

An Assay for Analyzing Test β-Glucan as an Immunotherapy Strategy against Brain Cancer Cells

A Method for the High Salt Treatment of Dendritic Cells and their Adoptive Transfer into Mice

Generating Single Positive CD8 T Cells from Induced Pluripotent Stem Cells

Model Development and Disease Study

A Near-Infrared Photoimmunotherapy Technique to Treat Lung Cancer in a Murine Model

Isolating Cord Blood Hematopoietic Stem Cells to Generate Human Immune System Mice

An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor

Oral Combinational Antiretroviral Therapy to Treat HIV-1 Infection in a Humanized Mouse Model

A Technique for Human Skin Grafting in a Mouse Model

A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model

Intracranial Cannular Implantation in a Mouse to Deliver Therapeutic Agents to Brain Tumors

Targeting Liver Tumors with Oncolytic Viruses via the Hepatic Artery

Evaluating the Anti-Tumor Efficacy of a Bacterial Immunomodulatory Protein in a Murine Model

A Cell-Based Therapy for Autoimmune Arthritis in a Murine Model

Delivering Effector T Lymphocytes into Mice Carrying Human Brain Tumors

Generating a Double Humanized BLT (Bone-Marrow, Liver, Thymus) Mouse Model

A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs

A Mouse Aortic Interposition Model to Study Alloimmune-Induced Vascular Rejection

A Mouse Model of Vascularized Heterotopic Spleen Transplantation

Generating the Widespread Cerebral Cortical Demyelination in a Rat Model

Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen

Acufection-Mediated Delivery of Immune-Related Genes into Mouse Skin Tissues

Evaluating the Effects of Fecal Microbiota Transplantation in an Interleukin-10 Deficient Mouse

Developing a Humanized Mouse Model with Human Liver Cells and Immune Cells

A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos

A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat

Constructing a Humanized Mouse Model with Engineered Immunity against HIV

Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System

Canalostomy for Local Drug Delivery into the Inner Ear of an Adult Mouse

Imaging Transgenic Beta-Cells in an Immunodeficient Mouse Challenged with Diabetic Splenocytes

Immunogenics and Vaccine Development

Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model

Generating a Multivalent-Displaying Outer Membrane Vesicle Vaccine

Preparation of a Nanoemulsion Adjuvant Vaccine

Intranasal Immunization with BCG in a Mouse Model

Generating Recombinant Live-Attenuated Influenza Viruses for Vaccines

A Technique to Generate a Novel Delivery Platform for a Delayed Burst Release of Molecules

An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant

Formulating mRNA-Loaded Nanoparticles for Vaccination Purposes

Purification of Self-Assembling Protein Nanoparticles using Affinity Chromatography

An Ultrasound-Guided Injection of Therapeutic Drugs into Human Salivary Glands

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG

An In Vitro Assay for Screening Interferons as Potent Inhibitors of Zika Virus Growth

Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model