Encyclopedia Of Experiments

Skin Cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Encyclopedia Of Experiments

Skin Cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Sections

Assays and techniques

Animal model studies

Assays and techniques

Animal model studies

Assays and techniques

HCR-DNA FISH

A Fluorescence In Situ Hybridization Technique to Detect Viral DNA in Infected Cells

Recombinant Merkel Cell Polyomavirus (MCPyV) Virion Preparation

A Technique to Produce High-titer Recombinant MCPyV Virion

Keratinocyte Colony Formation Assay

An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies

Tumor Cell Invasion Assay

A 3D In Vitro Native Matrix-based Method to Assess Epithelial-mesenchymal Cell Interactions

Organotypic 3D Skin Culture

A Cassette-based In Vitro Culture Technique to Regenerate Human Epidermis on Acellular Dermis

Hanging Drop Method

A Technique to Generate 3D Melanoma Spheroids

Human Skin Keratinocyte Isolation

A Method to Culture Primary Keratinocytes from Skin Samples

Genetic Modification of Primary Human Keratinocytes

A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses

Melanoma Stem Cell Isolation

A Method to Obtain CD133 Positive and Negative Melanoma Cells Using Magnetic-activated Cell Sorting

CUBIC-based Protein Expression Visualization

A Procedure for Visualizing Protein Expression in Full Thickness Mouse Skin Biopsies

Generation of Organotypic Full Skin Reconstructs

A Procedure to Establish 3D Skin Model Using Human Skin Samples

Frozen Skin Tissue Block Preparation

A Protocol to Preserve Cutaneous Melanoma Samples from Murine Dorsal Skin

Rapid Culturing of Primary Murine Melanocytes and Fibroblasts

Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample

Antibody Staining of Scale Melanocytes

A Technique to Detect Transcription Factors Expressed in Dorsal Scale-associated Melanocytes of Zebrafish Models

Animal model studies

Chemical-induced Two-stage Skin Carcinogenesis Model

An Experimental In Vivo Mouse Model of Skin Cancer

Experimental Metastatic Melanoma Mouse Model

A Platform to Study the Metastatic Potential of Melanoma Cells in the Lungs

Subcutaneous Transplantation Assay

A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model

miniCoopR Vector-based Transgenesis

A Technique to Screen Melanoma-inducing Genes in Zebrafish Tumor Model

Minimum Erythematous Dose Assay

A Method for Measuring UV Sensitivity in Mouse Models

Patient-derived Xenograft Modeling

A Technique to Generate Melanoma Mouse Models