Chinese PLA General Hospital 5 articles published in JoVE Medicine A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts Xiaoyi He1,2, Libing Li1, Wenhong Xu1, Shengli Jiang1,2 1Cardiovascular Surgery, First Medical Center, Chinese PLA General Hospital, 2Medical School of Chinese PLA This protocol presents a model of long-term ventricular fibrillation in rat hearts induced by continuous stimulation with low-voltage alternating current. This model has a high success rate, is stable, reliable, and reproducible, has a low impact on cardiac function, and causes only mild myocardial injury. Biology A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model Siting Zhang*1, Zeyu Cai*1, Xu Zhang1, Tianfeng Ma2, Wei Kong1 1Department of Physiology and Pathophysiology, School of Basic Medical Sciences; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University, 2Department of Vascular and Endovascular Surgery, the First Medical Centre, Chinese PLA General Hospital This protocol describes a calcium phosphate-induced abdominal aortic aneurysm (AAA) mouse model to study the pathological features and molecular mechanisms of AAAs. Genetics Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform Chengming Xu*1, Riqing Wei*2, Hui Lin1, Leiyu Deng1, Li Wang3, Deyang Li4, Honghui Den5, Wensong Qin1, Ping Wen1, Ying Liu1, Yingsong Wu2, Qiang Ma2, Jinliang Duan1 1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd. The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results. Medicine The Miniature Pig: A Large Animal Model for Cochlear Implant Research Xiaojun Ji*1,2,3,4, Yi Luo*1,2,3,4, Weiwei Guo1,2,3,4, Fei Ji1,2,3,4, Shuolong Yuan1,2,3,4, Liangwei Xu1,2,3,4, Wei Chen*1,2,3,4 1College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, 2National Clinical Research Center for Otolaryngologic Diseases, 3Key Lab of Hearing Science, Ministry of Education, 4Beijing Key Lab of Hearing Impairment for Prevention and Treatment Miniature pigs (mini-pigs) are an ideal large animal model for research into cochlear implants. Cochlear implantation surgery in mini-pigs can be utilized to provide initial evidence of the safety and potential performance of novel electrode arrays and surgical approaches in a living system similar to human beings. Immunology and Infection In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging Yucheng Wang1,2,3, Olivia D. Harrington1, Ying Wang1, Clinton K. Murray4, Michael R. Hamblin1, Tianhong Dai1 1Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, 2Department of Medical Oncology, Beijing Institute of Translational Medicine, Chinese Academy of Sciences, 3Cancer Center, Aviation General Hospital, Beijing, 4Infectious Disease Service, Brooke Army Medical Center Infections caused by multidrug-resistant (MDR) bacterial strains have emerged as a serious threat to public health, necessitating the development of alternative therapeutics. We present a protocol to evaluate the effectiveness of antimicrobial blue light (aBL) therapy for MDR Acinetobacter baumannii infections in mouse burns by using bioluminescence imaging.
