Anhui Medical University 4 articles published in JoVE Biochemistry Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages Jiale Cheng1,2, Jiong Zhu1,2, Yujiao Liu2,3, Chen Yang2,3, Yuehui Zhang2, Yuchen Liu2, Chaozhi Jin2, Jian Wang1,2 1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry. Chemistry Constructing Cyclic Peptides Using an On-Tether Sulfonium Center Chunli Song*1, Zhanfeng Hou*1,2, Zijun Jiao*1, Zhaodi Liu3, Chenshan Lian1,2, Min Zhang1, Wei Liang3, Feng Yin1,2, Zigang Li1,2 1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center. Cancer Research Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro Jinsen Lu1, Lele Wu2, Xiaoke Wang2, Jinhang Zhu2, Juan Du2, Bing Shen2 1School of Basic Medical Sciences, Anhui Medical University, 2Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University Here we present a detailed protocol for the application of rhodamine 123 to identify the mitochondrial membrane potential (MMP) and study CLIC4 knockdown-induced HN4 cell apoptosis in vitro. Under common fluorescence microscope and confocal laser scanning fluorescence microscope, the real-time change of the MMP was recorded. Medicine Ultrasonography in Experimental Reproductive Investigations on Rats Tianjuang Wang1,2, Lidia Oltra-Rodríguez3, Nuria García-Carrillo3, Anibal Nieto4,5, Yunxia Cao*1,2,6, María L. Sánchez-Ferrer*4,5 1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, 2Institute of Reproductive Genetics, Anhui Medical University, 3Centro Experimental en Investigaciones Biomédica (CEIB), Campus Ciencias de la Salud, Universidad de Murcia, 4Department of Obstetrics and Gynecology, 'Virgen de la Arrixaca' University Clinical Hospital, 5Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 6Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs This manuscript describes the utility of ultrasound performed on female rats to design experimental models for reproductive and gynecological investigation. A step-by-step explanation of how to perform ultrasonographic evaluation is shown.
