Shenzhen Bay Laboratory 3 articles published in JoVE Cancer Research Spontaneous Murine Model of Anaplastic Thyroid Cancer Huayun Yan1, Yingfang Ma1, Xinyue Zhou2, Yushuang He3, Yang Liu2, Carlos Caulin4, Leiming Wang5, Heng Xu1,6, Han Luo2,6 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, 2Division of Thyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-Related Molecular Network, Department of General Surgery, West China Hospital, Sichuan University, 3Department of Ultrasound, West China Hospital of Sichuan University, Sichuan University, 4Department of Otolaryngology-Head & Neck Surgery and University of Arizona Cancer Center, University of Arizona, 5Center for Translational Medicine, Shenzhen Bay Laboratory, 6Division of Laboratory Medicine/Research Centre of Clinical Laboratory Medicine, West China Hospital, Sichuan University Here, we present a standard pipeline to obtain murine ATC tumors by spontaneous genetically engineered mouse models. Further, we present tumor dynamics and pathological information about the primary and metastasized lesions. This model will help researchers to understand tumorigenesis and facilitate drug discoveries. Chemistry Constructing Cyclic Peptides Using an On-Tether Sulfonium Center Chunli Song*1, Zhanfeng Hou*1,2, Zijun Jiao*1, Zhaodi Liu3, Chenshan Lian1,2, Min Zhang1, Wei Liang3, Feng Yin1,2, Zigang Li1,2 1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center. Chemistry Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry Lingxiao Chaihu1,2, Xiaopeng Yao1, Xiang Xu1,2, Zhongqin Zhu1, David D. Y. Chen3, Guanbo Wang1 1School of Chemistry and Materials Science, Nanjing Normal University, 2Institute for Cell Analysis, Shenzhen Bay Laboratory, 3Department of Chemistry, University of British Columbia Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.
