Institute for Drug Discovery Innovation 1 article published in JoVE Neuroscience Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons Noriko Koganezawa1, Reiko T. Roppongi2, Yuko Sekino3,4, Izuo Tsutsui3, Ayaka Higa5, Tomoaki Shirao1,5 1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc. A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.
