Yonsei University View Institution's Website 12 articles published in JoVE Behavior A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay Saebom Kwon1, Jin I. Lee2, Kyoung-hye Yoon1 1Department of Physiology, Mitohormesis Research Center, Yonsei University Wonju College of Medicine, 2Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Mirae Campus This article describes a simple, low-cost method of recording the lawn avoidance behavior of Caenorhabditis elegans, using readily available items such as a smartphone and a light emitting diode (LED) light box. We also provide a Python script to process the video file into a format more amenable for counting. Engineering Measuring the Complete-arch Distortion of an Optical Dental Impression Ji-Man Park1, June-Sung Shim1 1Department of Prosthodontics, College of Dentistry, Yonsei University Here, we present a protocol to measure the degree of distortion at each part of the compete-arch digital impression acquired from an intraoral scanner with 3D-printed metal phantom with standard geometries. Biology An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay Joo Hye Yeo1, Jinu Lee1 1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment. Neuroscience Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue Jiwon Woo1,2,3, Eunice Yoojin Lee4, Hyo-Suk Park1,3, Jeong Yoon Park1,3, Yong Eun Cho1,2,3 1Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 3The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital, Yonsei University College of Medicine, 4Columbia University College of Physicians and Surgeons Here, we present two novel methodologies, psPACT and mPACT, for achieving maximal optical transparency and subsequent microscopic analysis of tissue vasculature in the intact rodent whole CNS. Environment Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation Hosub Lee1, Youn-Tae Kim2, Seulki Jeong1, Hye-On Yoon1 1Korea Basic Science Institute, Seoul Center, 2Natural Science Research Institute, Yonsei University This article presents modified experimental protocols for dimethylmonothioarsinic acid (DMMTAV) and dimethyldithioarsinic acid (DMDTAV) synthesis, inducing dimethylarsinic acid (DMAV) thiolation through mixing of DMAV, Na2S, and H2SO4. The modified protocol provides an experimental guideline, thereby overcoming limitations of the synthesis steps that could have caused experimental failures in quantitative analysis. Neuroscience Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury MinGi Kim1,2, Ji Hea Yu1, Jung Hwa Seo1,2, Yoon-Kyum Shin1,2, Soohyun Wi1,2, Ahreum Baek1,3, Suk-Young Song1,5, Sung-Rae Cho1,2,4,5 1Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 3Department of Rehabilitation Medicine, Yonsei University Wonju College of Medicine, 4Rehabilitation Institute of Neuromuscular Disease, Yonsei University College of Medicine, 5Graduate Program of NanoScience and Technology, Yonsei University We performed unilateral carotid artery occlusion on postnatal day 7-10 CD-1 mouse pups to create a neonatal hypoxic-ischemic (HI) model and investigated the effects of HI brain injury. We studied neurobehavioral functions in these mice compared to non-operated normal mice. Biochemistry Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG Meong Il Kim1, Choongdeok Lee1, Minsun Hong1 1Division of Biological Science and Technology, Yonsei University Bacillus anthracis is the obligate pathogen of fatal inhalational anthrax, so studies of its genes and proteins are strictly regulated. An alternative approach is to study orthologous genes. We describe biophysicochemical studies of a B. anthracis ortholog in B. cereus, bc1531, requiring minimal experimental equipment and lacking serious safety concerns. Medicine Evaluation of Drug Sorption to PVC- and Non-PVC-based Tubes in Administration Sets Using a Pump Su-Eon Jin1,2,3, Siwon You1,3, Seungho Jeon4, Hyo-Jin Byon2, Sung-Joo Hwang1,3 1College of Pharmacy, Yonsei University, 2College of Medicine, Yonsei University, 3Yonsei Institute of Pharmaceutical Sciences, 4Polyscientech Co. Ltd We report a method for drug sorption evaluation using a pump and model drugs (diazepam and tacrolimus). After drug analysis using high-performance liquid chromatography, drug concentrations and sorption levels in the tubes of administration sets are calculated. Biology Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory Tong Y. Lee1, Kyoung-hye Yoon1, Jin I. Lee1 1Division of Biological Science and Technology, Yonsei University We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates. Bioengineering A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering Uihan Kim1, Jaewoo Song2, Suho Ryu1, Soocheol Kim1, Chulmin Joo1 1School of Mechanical Engineering, Yonsei University, 2Department of Laboratory Medicine, Yonsei University A photo-thermal angular light scattering (PT-AS) sensor enables the rapid and chemical-free hemoglobin assay of nanoliter-scale blood samples. Here, details of the PT-AS setup and a measurement protocol for the hemoglobin concentration in blood are provided. Representative results for anemic blood samples are also presented. Immunology and Infection Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice Hyo Jin Park1, Ji Hoon Oh1, Sang-Jun Ha1 1Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells. Biology Thin Sectioning of Slice Preparations for Immunohistochemistry Jae-Joon Park1,2, Miles G. Cunningham2 1Department of Medicine, Yonsei University College of Medicine, Severance Hospital, 2Department of Psychiatry, Harvard Medical School The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.