VIB-UGent 2 articles published in JoVE Biology Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy Christopher J. Guérin1,2,3, Anna Kremer1,2,3, Peter Borghgraef1,2,3, Saskia Lippens1,2,3 1VIB Bio Imaging Core, 2VIB Inflammation Research Center, 3Department of Molecular Biomedical Research, UGent Here, we present a protocol for efficiently combining serial block face and focused ion beam scanning electron microscopy to target an area of interest. This allows for efficient searching, in three dimensions, and locating rare events in a large field of view. Immunology and Infection Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration Lien Van Hoecke1,2, Emma Richelle Job1,2, Xavier Saelens1,2, Kenny Roose1,2 1UGent Center for Medical Biotechnology, VIB, 2Department of Biomedical Molecular Biology, Ghent University (UGent) The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
