University of Murcia 4 articles published in JoVE Biology Autofluorescence Imaging to Evaluate Red Algae Physiology Teresa Coronado-Parra1, Mónica Roldán2,3, Marina Aboal4 1Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, 4Algology Laboratory, Biology Faculty, University of Murcia The present protocol describes step-by-step autofluorescence imaging and evaluation of phycobiliprotein changes in red algae based on spectral analysis. This is a label-free and non-destructive method to evaluate cellular adaptation to extreme habitats, when only scarce material is available and cells grow slowly, or not at all, under laboratory conditions. Medicine External Cephalic Version: Is it an Effective and Safe Procedure? Javier Sánchez-Romero1, Vanesa García-Soria2, Fernando Araico-Rodríguez1, Javier Herrera-Giménez1, José E. Blanco-Carnero1,3, Aníbal Nieto-Díaz1,3,4, María Luisa Sánchez-Ferrer1,3,4 1Department of Obstetrics & Gynecology, "Virgen de la Arrixaca", University Clinical Hospital, 2Department of Gynecology, "Reina Sofía", University Clinical Hospital, 3Department of Obstetrics and Gynecology, Pediatrics and Surgery, University of Murcia, 4Institute for Biomedical Research of Murcia This article shows how to perform the external cephalic version (ECV) by two experienced obstetricians in the obstetric operating room in the presence of an anesthesiologist and a midwife. The ECV is carried out with analgesia and tocolysis. Two attempts are made under ultrasonography control. Medicine Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing Sergio Liarte*1, Ángel Bernabé-García*1, David Armero-Barranco2, Francisco José Nicolás1 1Laboratorio de Oncología Molecular y TGF-β, IMIB-Arrixaca, 2Departamento de Enfermería, Facultad Enfermería, Universidad de Murcia This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration. Developmental Biology Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization María Jesús Sánchez-Calabuig1,2, Francisco Alberto García-Vázquez3, Ricardo Laguna-Barraza1, Carlos Barros-García4, Daniel García-Parraga5, Dimitrios Rizos1, Alfonso Gutiérrez Adan1, José Félix Pérez-Gutíerrez2 1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5 Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.
