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Biology
从AE肠和唾液腺的解剖。埃及伊蚊蚊

Research Article

从AE肠和唾液腺的解剖。埃及伊蚊蚊

DOI: 10.3791/228

July 4, 2007

Judy Coleman1, Jennifer Juhn1, Anthony A. James2

1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

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In This Article

Summary Abstract Introduction Protocol Representative Results Discussion Disclosures Acknowledgements Materials References Reprints and Permissions

Erratum Notice

Important: There has been an erratum issued for this article. View Erratum Notice

Retraction Notice

The article Assisted Selection of Biomarkers by Linear Discriminant Analysis Effect Size (LEfSe) in Microbiome Data (10.3791/61715) has been retracted by the journal upon the authors' request due to a conflict regarding the data and methodology. View Retraction Notice

Summary

蚊子中肠,唾液腺像恶性疟原虫疟疾和登革热病毒载体病原体的进入和退出的关键点。本视频演示了为消除由埃及伊蚊的蚊子的中肠,唾液腺的解剖技术。

Abstract

蚊子中肠,唾液腺中疟原虫的寄生虫病和登革热病毒等病原体的进入和退出的关键点。这个视频协议演示去除埃及伊蚊的蚊子的中肠和唾液腺的解剖技术。

Protocol

解剖中肠和蚊子的唾液腺组织需要事前准备1X磷酸盐缓冲液(1X PBS)的解决方案和蚊子麻醉承受温度4 ° C,直到固定。蚊子仍然放置在培养皿中,是保存在冰冷的的麻醉。其他所需材料包括:光镜下装有10倍的目标,移液器,细尖的镊子,载玻片,针尖探针。

肠夹层

  1. 放置一个安装在光学显微镜下的载玻片上的1X PBS下​​降。
  2. 通过刺针探针蚊子的胸部转移到准备好的幻灯片一种通过蚊子传播的。
  3. 按住蚊子探头,使用镊子把握最后腹节的第二个和轻轻地拉过蚊子的腹部在一个单一的议案。中肠应保持连接到固定胸部。
  4. 丢弃的腹部。使用镊子脱离胸部中肠。

涎腺淋巴清扫术

  1. 将一个光显微镜下安装在玻片上的1X PBS下​​降。
  2. 拿起针探针stabbling胸部一种通过蚊子传播的。
  3. 拉断蚊子腿,用手指。
  4. 转移到幻灯片的蚊子。
  5. 取出使用产钳的蚊子的头部。
  6. 按住蚊子的胸部,用探针使用另一个探头轻轻推胸部。
  7. 唾液腺位于胸廓的前部,可以通过使用探针针尖和切断腺体连接到胸部的附件隔离。完整的唾液腺是由三个叶:两个侧叶和内侧叶。

Disclosures

蚊子中肠,唾液腺像恶性疟原虫疟疾和登革热病毒载体病原体的进入和退出的关键点。本视频演示了为消除由埃及伊蚊的蚊子的中肠,唾液腺的解剖技术。

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从AE肠和唾液腺的解剖。埃及伊蚊蚊
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