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JoVE Journal > Immunology and Infection
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Summary
Abstract
Introduction
Protocol
Results
Discussion
Disclosures
Acknowledgements
Materials
References
Immunology and Infection
口咽部气管内PAMP管理和支气管肺泡灌洗的利用率来评估小鼠宿主的免疫反应
Published: April 02, 2014
doi:
10.3791/51391
Please note that all translations are automatically generated.
Click here for the English version.
Irving C. Allen
1
1
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine
,
Virginia Polytechnic Institute and State University
Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
C57Bl/6J
The Jackson Laboratory
Stock 000664
Compact Scale
Ohaus Scale Corporation
71142845
Small Animal Rectal Thermometer
Braintree Scientific
TH 5
Rectal Probe for Rodents
Braintree Scientific
RET 3
Ear Punch
Braintree Scientific
EP-S 901
Lipopolysaccharide from <em>E. coli</em> 0111:B4
InvivoGen
LPS-EB
1x Phosphate Buffered Saline
Life Technologies
10010-023
Isoflurane
Baxter
40032609
Intratrachael Administration and Lung Inflation Stand
ICAP Manufacturing
n/a
Rodent Intubation Stand
Braintree Scientific
RIS 100
Scissors (blunt/sharp)
Fisher Scientific
13-806-2
forceps (straight)
Fisher Scientific
22-327-379
forceps (45º, curved)
Fisher Scientific
10-275
Scissors (blunt/blunt)
Fisher Scientific
08-940
Pipette (200 µl Capacity)
Gilson
F123601
Ethanol
Sigma
459844
1 ml Syringe
BD Medical
301025
10 ml Syringe
BD Medical
301604
27 G x 0.5 in Needle
BD Medical
305109
Refrigerated Microcentrifuge
Fisher Scientific
13-100-676
1.2 mm Tracheal Cannulae with Luer-adapter
Harvard Apparatus
732836
Hank's Balanced Salt Solution
Life Technologies
14025-076
4-0 Silk Braided Surgical Suture
Ethicon
A183
Luer to Tube Connector Kits
Harvard Apparatus
721406
Luer Stopcock Kit
Harvard Apparatus
721664
Tygon formula E-3603 Laboratory Tubing
Sigma
R-3603
Formalin Solution, neutral buffered, 10%
Sigma
HT501128-4L
Mouse IL-1β OptEIA ELISA Kit
BD Biosciences
559603
Mouse IL-6 OptEIA ELISA Kit
BD Biosciences
550950
Mouse TNF-α OptEIA ELISA Kit
BD Biosciences
560478
Hemocytometer
Hausser Scientific
3520
Hemocytometer Cover Glasses
Thermo Scientific
22-021-801
Trypan Blue
Thermo Scientific
SV3008401
Cytology Funnel Clips
Fisher Scientific
10-357
Cytology Funnels
Fisher Scientific
10-354
Filter Cards
Fisher Scientific
22-030-410
Microscope Slides
Fisher Scientific
12-544-1
Cover Glasses
Fisher Scientific
12-540A
Cytospin Cytocentrifuge
Thermo Scientific
A78300003
Diff Quick Staining Kit
Fisher Scientific
47733150
Permount Mounting Medium
Fisher Scientific
SP15-500
DOWNLOAD MATERIALS LIST
References
Matute-Bello, G., et al.
An official American Thoracic Society workshop report: features and measurements of experimental acute lung injury in animals.
Am. J. respir. Cell Mol. Biol
.
44
, 725-738 (2011).
Egger, C., et al.
Administration of bleomycin via the oropharyngeal aspiration route leads to sustained lung fibrosis in mice and rats as quantified by UTE-MRI and histology.
PloS one
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, (2013).
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Nonsurgical intratracheal instillation of mice with analysis of lungs and lung draining lymph nodes by flow cytometry.
J. Vis. Exp
. , (2011).
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A non-invasive intratracheal inoculation method for the study of pulmonary melioidosis.
Front. Cell. Infect. Microbiol
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, 164 (2012).
Allen, I. C., et al.
Analysis of NLRP3 in the development of allergic airway disease in mice.
J. Immunol
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, 2884-2893 (2012).
Allen, I. C., et al.
Characterization of NLRP12 during the in vivo host immune response to Klebsiella pneumoniae and Mycobacterium tuberculosis.
PloS one
. , (2013).
Allen, I. C., et al.
Expression and function of NPSR1/GPRA in the lung before and after induction of asthma-like disease.
Am. J. Physiol. Lung Cell. Mol. Physiol
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, 1005-1017 (2006).
Kebaier, C., et al.
Staphylococcus aureus alpha-hemolysin mediates virulence in a murine model of severe pneumonia through activation of the NLRP3 inflammasome.
J. Infect. Dis
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, 807-817 (2012).
Roberts, R. A., et al.
Analysis of the murine immune response to pulmonary delivery of precisely fabricated nano- and microscale particles.
PloS one
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8
, (2013).
Willingham, S. B., et al.
NLRP3 (NALP3, Cryopyrin) facilitates in vivo caspase-1 activation, necrosis, and HMGB1 release via inflammasome-dependent and -independent pathways.
J. Immunol
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183
, 2008-2015 (2009).
Kline, J. N., et al.
Variable airway responsiveness to inhaled lipopolysaccharide.
Am. J. Respir. Crit. Med
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160
, 297-303 (1999).
Cressman, V. L., Hicks, E. M., Funkhouser, W. K., Backlund, D. C., Koller, B. H.
The relationship of chronic mucin secretion to airway disease in normal and CFTR-deficient mice.
Am. J. Respir. Cell Mol. Biol
.
19
, 853-866 (1998).
Tags
Oropharyngeal Intratracheal Administration
Bronchoalveolar Lavage
Host Immune Response
Lipopolysaccharide
PAMP
Acute Lung Inflammation
Innate Immunity
Host-pathogen Interaction
Mouse Model
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Cite This Article
Allen, I. C. The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice.
J. Vis. Exp.
(86), e51391, doi:10.3791/51391 (2014).
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