-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

CN

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

zh_CN

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Biology
从AE肠和唾液腺的解剖。埃及伊蚊蚊
从AE肠和唾液腺的解剖。埃及伊蚊蚊
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

从AE肠和唾液腺的解剖。埃及伊蚊蚊

Full Text
36,059 Views
03:31 min
July 4, 2007

DOI: 10.3791/228-v

Judy Coleman1, Jennifer Juhn1, Anthony A. James2

1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

AI Banner

Please note that some of the translations on this page are AI generated. Click here for the English version.

蚊子中肠,唾液腺像恶性疟原虫疟疾和登革热病毒载体病原体的进入和退出的关键点。本视频演示了为消除由埃及伊蚊的蚊子的中肠,唾液腺的解剖技术。

我叫 Judy Coleman,是 James Lab 的一员。在 UCI,我们在分子生物学和生物化学系。今天,我将解剖 Eptide 蚊子,我们将解剖中肠的唾液腺。

我们对这些特定组织感兴趣,因为它们与病毒和寄生虫的途径密切相关。当蚊子摄入血粉时,寄生虫病毒会进入唾液腺,然后从唾液腺进入中肠。它穿过中肠上皮,然后进入嗡嗡淋巴液。

它通过蚊子的血液、血液淋巴液进入唾液腺。它在那里驻留,直到它准备好再吃一次血餐。好吧。

我现在首先要做的是剖析 80 年代埃及的中肠。首先,我需要穿上 1 次 PBS。这是一种缓冲,也就是我们良好的生理条件。

所以我们把它放在载玻片上,我感冒了,以防止蚊子在我们解剖时飞走,这样我就拿 ver midgut 来得到一个 midgut,我必须指向蚊子。我放上 my,我使用我制作的探针,然后将探针穿过蚊子的胸部。一旦我把它放在显微镜下,我拿起镊子,我拿起腹部吸力的末端,把它拉下来,留下中肠。

所以侏儒,侏儒就在那里,我会完成它。就在那里。有孤立的中肠。

现在,我们将解剖 80 年代 GT 眼的唾液腺。在这个过程中,我使用了两种不同的探针和镊子。我这里有一个探针,它有一个直针,我用它来抓住蚊子。

我使用一个带有斜针的探头,我用它来将 ate 镜头从蚊子中推出。在取出唾液腺之前,我用镊子将蚊子的头部拉下来。当我把蚊子从培养皿中取出时,我戳了戳它的胸部。

如果我能去掉腿,那么拉出腺体就更容易了。所以我只是手工做。然后我把蚊子放在缓冲器里,把她的头拉下来,推着胸部,然后把唾液腺推出来。

这是对唾液腺的一个很好的观察,也是对流涎腺的两个部分的观察。我们刚刚解剖完八十年代内脏中流涎的腺体,石膏眼蚊。我们在分子水平上观察这种材料,以干扰病毒的途径或蚊子内的寄生虫。

Explore More Videos

细胞生物学 第5期 蚊子 疟疾 解剖 传染病

Related Videos

在蚊子(埃及斑蚊)感染引起的表型测定为登革热感染病例的议定书"

15:25

在蚊子(埃及斑蚊)感染引起的表型测定为登革热感染病例的议定书"

Related Videos

19.1K Views

恶性疟原虫在蚊子和病毒感染的表型测定感染的议定书

14:10

恶性疟原虫在蚊子和病毒感染的表型测定感染的议定书

Related Videos

18.4K Views

杂交原位和蚊虫唾液腺,卵巢,胚胎

10:58

杂交原位和蚊虫唾液腺,卵巢,胚胎

Related Videos

17.2K Views

从蚊子中肠分离细菌

02:40

从蚊子中肠分离细菌

Related Videos

29 Views

啮齿动物疟原虫无性、性血期和蚊子阶段的类状分析

08:23

啮齿动物疟原虫无性、性血期和蚊子阶段的类状分析

Related Videos

12.2K Views

抗生素处理蚊子的细菌口服喂养测定

09:59

抗生素处理蚊子的细菌口服喂养测定

Related Videos

7.2K Views

恶性疟原虫 通过人工膜喂养进行配子体培养和蚊子感染

09:23

恶性疟原虫 通过人工膜喂养进行配子体培养和蚊子感染

Related Videos

8.4K Views

研究成年蚊子排泄系统神经肽和其他调节剂的活性

11:30

研究成年蚊子排泄系统神经肽和其他调节剂的活性

Related Videos

3.3K Views

冈 比亚按蚊 幼虫唾液腺的解剖和免疫染色

06:12

冈 比亚按蚊 幼虫唾液腺的解剖和免疫染色

Related Videos

5.2K Views

经口喂养和显微注射对成年蚊子进行实验性病毒感染

08:02

经口喂养和显微注射对成年蚊子进行实验性病毒感染

Related Videos

3K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code