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JoVE Encyclopedia of Experiments
Neuroscience
小鼠大脑中人类神经祖细胞的移植
小鼠大脑中人类神经祖细胞的移植
Encyclopedia of Experiments
Neuroscience
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Encyclopedia of Experiments Neuroscience
Transplantation of Human Neural Progenitor Cells in the Mouse Brain

小鼠大脑中人类神经祖细胞的移植

Protocol
71 Views
02:17 min
August 29, 2025
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Please note that some of the translations on this page are AI generated. Click here for the English version.

Transcript

将麻醉

的小鼠固定在立体定位框架中。

涂抹眼药膏以防止角膜干燥。

剃

掉头皮并消毒皮肤。

插入耳杆以稳定头骨。

然后,做一个中线切口以暴露头骨。

在

目标坐标处在头骨上钻一个洞。

将表达

荧光素酶和荧光蛋白的转基因人类神经祖细胞或 NPC 装入注射器以进行可视化。

将

注射器与目标部位对齐,并将针头降低到所需深度。

在

针头周围涂上组织粘合剂以防止细胞渗漏。

缓慢注射细胞以确保均匀分布。

将

针头短暂固定到位,以防止细胞悬液回流。

最后,慢慢拔出针头并闭合切口。

移植了 NPC 的小鼠模型现在可以可视化了。

将

动物从诱导室运送到立体定向框架,使用面罩保持麻醉。

涂抹眼科润滑剂以防止眼睛干燥。用电动剃须刀剃掉小鼠头皮,并使用棉签用 5% 甜菜碱溶液对皮肤进行消毒。固定鼠标头并将耳杆插入外鼻道。

用外科牙钻在头骨上钻一个直径为两到三毫米的孔。将准备好的细胞重悬于管中,并将两微升细胞悬液吸入注射器中。将注射器放在目标部位上方,慢慢将针头移动到硬脑膜表面并计算深度坐标。

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