宫内癌细胞注射:一种递送癌细胞以建立腹膜内卵巢癌小鼠模型的技术

宫内细胞注射允许将感兴趣的细胞递送到受体动物的子宫腔中。首先，在右侧位置准备麻醉的免疫功能低下的裸鼠。做一个手术切口并解剖腹肌以暴露其腹腔。

找到其中一个子宫角。这些管状结构类似于两个不同的子宫，从两侧的输卵管延伸并通向单个子宫颈。

接下来，夹住角的前端和后端进行分割。现在，将卵巢癌细胞悬液缓慢注射到分段的子宫腔中，确保细胞的高效输送。

随后，将子宫角放回腹腔并缝合手术切口。让鼠标在笼子里恢复。

注射后几天内，注射部位出现肿瘤，并转移在腹膜腔内的不同组织或器官中形成继发性肿瘤。

要开始此过程，请用2%异氟醚麻醉7至8周龄的无胸腺裸鼠。然后，通过捏住脚垫来验证它是否已完全麻醉。接下来，将动物右侧放在无菌纱布垫上，头部远离实验者。在双眼上涂抹药膏，以防止麻醉时干燥。

然后，快速将动物的头部插入连接到异氟醚蒸发器的鼻锥系统中。使用酒精垫对腹部右侧进行消毒，然后用碘消毒。然后，将无菌手术单放在上面。使用无菌手术剪刀和镊子，在小鼠的左下象限上做一个1至2厘米的皮肤切口。

之后，抬起肌肉并做一个切口以到达腹膜。随后，解剖斜肌以暴露腹腔。然后，找到左子宫角。使用无菌止血剂夹住角的前侧和后侧。

前夹放在输卵管正下方，将后夹放在子宫颈正上方。此时，让第二个实验者通过将含有针头的细胞垂直于角以 45 度角放置来注射细胞悬液。然后，将50微升细胞悬液缓慢注射到子宫角腔内。接下来，松开前夹，然后松开后

之后，将子宫角放回腹腔内。使用合成可吸收缝合线闭合腹膜。然后，使用纸巾粘合剂闭合皮肤。

完成后，将鼠标从鼻锥中取出，然后将其放回笼子中。在无人看管之前，请确保动物清醒且活跃。手术后的前 48 小时内在饮用水中提供布洛芬。

• Mouse Ovary – The egg-producing reproductive organ in female mice.
• Hemostat – A surgical tool used to control bleeding.
• Cell Injection – The process of inserting cells into a certain tissue or organ.
• Uterine Carcinoma – A type of cancer that affects the uterus.
• Oviducts - Tubes that link the ovaries to the uterus in female mammals.
• Uterine Horn - Part of the uterus in various animals, extending laterally to the Fallopian tubes.
• Forceps – A handheld hinged instrument used for grasping and holding objects.

• Introduce Mouse Ovary - Mouse ovaries produces eggs needed for reproduction (e.g., mouse ovary).
• Key Concepts - Summarizing principles like cell injection, and controlling bleeding (e.g., hemostat).
• Explain Underlying Mechanism - Brief overview of uterine carcinoma, a type of cancer affecting the uterus.
• Connect to Experiment - Link the background to the surgical study carried out on mice's ovary.

• What is cell injection and how is it done in mouse ovary?
• How are hemostats utilized in this surgical process?
• How does the uterine carcinoma affect mouse's reproductive system?

• Practical Applications – Mice surgeries provide vital information for human health (e.g., uterine carcinoma).
• Industry Impact - Could be useful to develop new treatments for humans (e.g., cell injection).
• Societal Importance - Aid understanding of human reproductive system (e.g., oviducts).
• Link to Scientific Advancements - Development in surgical methods and techniques for treatment (e.g., forceps).