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RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
JoVE 杂志
生物学
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JoVE 杂志 生物学
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

DOI:
10.3791/3479-v

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08:47 min

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July 14, 2012

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, , ,
1Biology Department,New Mexico State University, 2Molecular Biology Department,New Mexico State University

Chapters

  • 00:05Title
  • 01:48Injection Preparation
  • 03:07Mosquito Injection
  • 04:26Mosquito Recovery and Storage
  • 04:53In vivo Diuresis Assay
  • 05:32Injection Preparation
  • 06:16Mosquito Injection
  • 06:41Weighing Mosquitoes
  • 07:27Results
  • 07:51Conclusions

Summary

自动翻译

自动翻译

In this protocol we combine RNAi-mediated gene silencing with an in vivo diuresis assay to study the effects knockdown of genes of interest has on mosquito fluid excretion.

Tags

RNAi-mediated Gene KnockdownIn Vivo Diuresis AssayAedes Aegypti MosquitoesGene Knockdown TechniqueBioassayDiuresis In InsectsAquaporin GenesMosquito Injection DeviceDsRNA InjectionExcretion Rates

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