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Kombineret Immunofluorescens og DNA FISH på 3D-bevaret Interfasen Kerner til Uddannelse Ændringer i 3D Nuclear Organization

Article DOI: 10.3791/50087-v • 13:55 min • February 3rd, 2013
February 3rd, 2013 •
1, 1,2,3,4, 1
1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

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Her beskriver vi en protokol til samtidig påvisning af histon ændringer ved immunofluorescens og DNA sekvenser ved DNA FISK efterfulgt af 3D mikroskopi og analyser (3D immuno-DNA FISH).

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Genetik molekylærbiologi bioinformatik Cancer Biology Patologi Biomedical Engineering Immunology intranukleart Space Nuclear Matrix Fluorescens FISH 3D DNA FISH DNA immunfluorescens immuno-FISH 3D mikroskopi Nuclear organisation interfase kerner chromatin modifikationer
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