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Biology
分离,培养和移植肌卫星细胞
分离,培养和移植肌卫星细胞
JoVE Journal
Biology
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JoVE Journal Biology
Isolation, Culture, and Transplantation of Muscle Satellite Cells

分离,培养和移植肌卫星细胞

Full Text
43,126 Views
10:25 min
April 8, 2014

DOI: 10.3791/50846-v

Norio Motohashi1, Yoko Asakura1, Atsushi Asakura1

1Stem Cell Institute, Paul and Sheila Wellstone Muscular Dystrophy Center, Department of Neurology,University of Minnesota Medical School

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Please note that some of the translations on this page are AI generated. Click here for the English version.

静止卫星细胞的纯群体的分离和培养,肌肉干细胞群,是必不可少的肌肉干细胞生物学和再生,以及干细胞移植在肌营养不良症和其他退行性疾病的治疗方法的理解。

该程序的总体目标是移植纯化的、静止的成人神经元骨骼肌卫星细胞,用于治疗骨骼肌损伤。这是通过首先解剖成年小鼠的骨骼肌并酶消化骨骼肌,然后通过磁珠分离对静止的卫星细胞进行分类来实现的。接下来,将分离的细胞在胶原蛋白包被的培养皿上扩增,最后一步,将体外扩增的细胞注射到成年小鼠的 Cardio toin 处理的骨骼肌中。

最终,可以通过 xal 染色评估体外扩增卫星细胞的植入和对肌肉纤维再生的贡献。嗨,我的名字是寿司 ura。我是明尼苏达大学干细胞研究所的副教授。

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