Recovery of Adult Zebrafish Hearts for High-throughput Applications

Rima Arnaout; Sven Reischauer; Didier Y.R. Stainier

doi:10.3791/52248

Journal

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生物学

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恢复成年斑马鱼的心脏的高通量应用

Recovery of Adult Zebrafish Hearts for High-throughput Applications

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生物学

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JoVE 杂志生物学

Recovery of Adult Zebrafish Hearts for High-throughput Applications

恢复成年斑马鱼的心脏的高通量应用

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12,248 Views

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06:51 min

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December 12, 2014

DOI:

10.3791/52248-v

DOI:

10.3791/52248-v

•

06:51 min

December 12, 2014

•

2 Views

Rima Arnaout¹, Sven Reischauer^2,3, Didier Y.R. Stainier^2,3

¹Cardiovascular Research Institute and Division of Cardiology,University of California San Francisco, ²Department for Biochemistry and Biophysics,University of California San Francisco, ³Max-Planck Institute for Heart and Lung Research

Summary

Use of zebrafish for cardiovascular research is expanding towards research on adult hearts. For these applications, quick and simple isolation of cardiac tissues is key to avoid post-mortem changes and to obtain an adequate number of samples. Here, we describe a fast and reproducible method for dissecting adult zebrafish hearts.