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Measurement of Larval Activity in the Drosophila Activity Monitor
JoVE 杂志
生物学
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JoVE 杂志 生物学
Measurement of Larval Activity in the Drosophila Activity Monitor
DOI:

09:30 min

April 30, 2015

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Chapters

  • 00:05Title
  • 01:11Setting Up the DAM Software
  • 02:34Preparations
  • 03:42Setting Up the Assay
  • 04:56Measuring Locomotion
  • 06:18Preparing and Processing the Data
  • 07:57Temperature Regulation of Locomotion, Locomotion of Mutant Larvae, Locomotion at Three Instar Stages and Consistency of Locomotion
  • 09:04Conclusion

Summary

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This report describes a method for measuring Drosophila larval activity using the TriKinetics Drosophila Activity Monitor. The device employs infrared beams to detect movements of up to 16 individual animals. Data can be analyzed to represent motion parameters including rates and the positions of the animals within the assay chambers.

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