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Dérivation libre Feeder-de mélanocytes de pluripotentes humaines Cellules souches

Article DOI: 10.3791/53806-v • 12:21 min • March 3rd, 2016
March 3rd, 2016 •
1,2, 3, 1
1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

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Ce travail décrit un protocole de différenciation in vitro pour produire pigmentées, mélanocytes matures à partir de cellules souches pluripotentes humaines par une crête neurale et mélanoblaste étape intermédiaire en utilisant un protocole de 25 jours sans alimentation.

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Developmental Biology numéro 109 mélanocytes pigmentation mélanoblaste cellules souches embryonnaires (CSE) les cellules souches pluripotentes humaines (hPSCs) induite par cellules souches pluripotentes (CISP) la crête neurale la différenciation in vitro modélisation des maladies protocole de différenciation embryonnaires humaines cellules souches
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