Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Joel J. Thevarajah; Michael D. O'Connor; Patrice Castignolles; Marianne Gaborieau

doi:10.3791/54549

Journal

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化学

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毛细管电泳监视肽接枝壳聚糖膜实时

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

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化学

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JoVE 杂志化学

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

毛细管电泳监视肽接枝壳聚糖膜实时

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9,036 Views

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11:05 min

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October 26, 2016

DOI:

10.3791/54549-v

DOI:

10.3791/54549-v

•

11:05 min

October 26, 2016

•

9020 Views

Joel J. Thevarajah^1,2,3, Michael D. O’Connor^1,4, Patrice Castignolles^2,3, Marianne Gaborieau^1,2,3

¹Molecular Medicine Research Group,Western Sydney University ²Australian Centre for Research on Separation Science,Western Sydney University ³School of Science and Health,Western Sydney University ⁴School of Medicine,Western Sydney University

Summary

Free solution capillary electrophoresis is a fast, cheap and robust analytical method that enables the quantitative monitoring of chemical reactions in real time. Its utility for rapid, convenient and precise analysis is demonstrated here through analysis of covalent peptide grafting onto chitosan films for improved cell adhesion.