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JoVE Journal
Medicine
肿瘤进展和血管生成的双生物发光成像
肿瘤进展和血管生成的双生物发光成像
JoVE Journal
Medicine
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JoVE Journal Medicine
Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

肿瘤进展和血管生成的双生物发光成像

Full Text
8,817 Views
10:56 min
August 1, 2019

DOI: 10.3791/59763-v

Kaiyue Zhang1, Chen Wang1, Ran Wang2, Shang Chen1, Zongjin Li1

1Nankai University School of Medicine, 2State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy,Nankai University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

该协议描述了通过双生物发光成像实时监测肿瘤进展和血管生成情况的肿瘤携带小鼠模型。

血管生成在肿瘤转移和进展中起着重要作用。在该协议中,我们将使用双生物发光成像方法监测乳腺癌小鼠模型中的动态肿瘤进展和血管生成。在此模型中,我们可以分别通过萤火虫荧光酶和雷尼拉荧光酶成像,在单个小鼠中同时跟踪肿瘤生长和血管发生。

该模型可广泛应用于抗肿瘤药物筛选和肿瘤学研究。这种双生物发光模型可用于跟踪肿瘤进展和回归,并检测肿瘤相关的分子过程,以响应治疗策略。血管生成是肿瘤进展的一个关键过程。

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