Perfusion and Inflation of the Mouse Lung for Tumor Histology

Mackenzie  L. Davenport; Taylor  P. Sherrill; Timothy  S. Blackwell; Mick D. Edmonds

doi:10.3791/60605

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Perfusion and Inflation of the Mouse Lung for Tumor Histology

肿瘤病理学鼠肺的灌注和膨胀

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04:24 min

August 6, 2020

DOI: 10.3791/60605-v

Mackenzie L. Davenport¹, Taylor P. Sherrill², Timothy S. Blackwell², Mick D. Edmonds¹

¹Department of Genetics,University of Alabama at Birmingham, ²Department of Medicine, Division of Allergy, Pulmonary, and Critical Care Medicine,Vanderbilt University School of Medicine

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该方法旨在为小鼠肺的灌注、膨胀和固定提供一种简单高效的方法，用于检查肺肿瘤病理学和对肺转移的评价。

演示该程序的是 Edmonds 实验室的研究生 Mackenzie Davenport。对小鼠实施安乐死后，用手术剪刀在下腹部中间做一个 3.5 到 5 毫米的水平切口。接下来，将手术剪刀插入切口，沿着中心中线垂直切割到鼠标颈部下方。

用手指将皮肤向后拉，检查腋窝淋巴结。使用手术剪刀做一个 3.5 毫米的横向切口以打开腹腔。然后沿内部方向切割至胸部底部。

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癌症研究第 162 期肺癌小鼠肺膨胀灌注固定肺肿瘤组织学肺转移