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微流体设备中的电场诱导神经前体细胞分化
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices
DOI:

07:15 min

April 14, 2021

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Chapters

  • 00:05Introduction
  • 00:46Design and Fabrication of the Multichannel, Optically Transparent, Electrotactic Chip and Coating Poly-L-lysine on the Substrate in the Cell Culture Regions
  • 02:49Coating Poly-L-lysine on the Substrate in the Cell Culture Regions and Preparation of the Salt Bridge Network
  • 04:47Setup of the Microfluidic System for DC Pulse Stimulation
  • 05:55Results: Differentiation of the mNPC Cells in the Control Group and in the DC Pulse Stimulation Group at DIV 3, 7, and 14
  • 06:43Conclusion

Summary

自动翻译

在这项研究中,我们提出了一个单纯由微流系统中直接电流(DC)脉冲刺激诱导的神经干细胞和祖细胞(NPC)分化的协议。

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