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Journal / Cancer Research / Kollajen Bazlı İskeleler Kullanılarak…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Kollajen Bazlı İskeleler Kullanılarak Nöroblastomun Üç Boyutlu İn Vitro Biyomimetik Modeli
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Kollajen Bazlı İskeleler Kullanılarak Nöroblastomun Üç Boyutlu İn Vitro Biyomimetik Modeli

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Bu makale, daha önce tarif edilen üç boyutlu kollajen bazlı iskelelerde nöroblastom hücre hatlarını tohumlamak, önceden belirlenmiş bir zaman dilimi için hücre büyümesini sürdürmek ve çeşitli hücre büyümesi ve hücre davranışı analizleri ve aşağı akış uygulamaları için iskeleleri almak için gereken adımları listeler.

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Üç Boyutlu İn Vitro Biyomimetik Model Nöroblastom Kollajen Bazlı İskeleler Pediatrik Kanser Tümör Dokusu Ekstraselüler Matriks (ECM) Hastalığın İlerlemesi Hasta Prognozu Terapötik Yanıt Nöroblastom Hücre Hatları Nanohidroksiapatit (nHA) Glikozaminoglikanlar (GAG'ler) Kemik ve Kemik İliği Metastatik Bölgeler Gözenekli Yapı Hücre Bağlanması Proliferasyon Migrasyon Hücre Kümeleri Terapötikler In Vivo Durum İskele Tabanlı Kültür Sistemi İki Boyutlu (2D) Hücre Kültür DNA Miktar Tayini Hücre Canlılığı
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