<em>In Vivo</em> Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

Stephanie L. Daugherty; Keara M. Healy; Jonathan  M. Beckel

doi:10.3791/64227

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In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

体内啮齿动物膨胀诱发尿路上皮ATP释放的管腔测量

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09:17 min

September 7, 2022

DOI: 10.3791/64227-v

Stephanie L. Daugherty¹, Keara M. Healy¹, Jonathan M. Beckel¹

¹Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine

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该协议描述了在麻醉啮齿动物中测量膀胱腔中ATP浓度的程序。

我们的协议测量ATP，这是膀胱中的重要递质，这将使我们能够进一步询问控制其释放的机制。我们的技术直接从膀胱腔测量ATP，使其比使用培养细胞或膀胱组织的技术更具生理相关性。演示该程序的将是我实验室的研究技术人员Keara Healy和Stephanie Daugherty。

首先，通过将动物置于用4%至5%异氟醚在氧气中气体的封闭盒中来诱导初始麻醉。皮下注射双侧聚氨酯，然后将动物放在笼子中，让聚氨酯生效近两个小时。等待聚氨酯生效后，用镊子捏住动物的脚来测试适当的麻醉平面。

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