Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing<em> In Vitro </em>Fertilization

Dilan Gokyer; Natasha Salpeter; Lydia Hughes; Hoi Chang Lee; Meredith J Lohman; Tomiris Atazhanova; Anna Kleinhans; Joan K Riley; MaryEllen Pavone; Francesca E Duncan; Emily Zaniker-Gomez; Elnur Babayev

doi:10.3791/69122

JoVE Journal
Medicine

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JoVE Journal Medicine

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization

从接受体外受精的个体收集人卵泡液、卵泡体细胞和未成熟卵母细胞

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06:40 min

October 24, 2025

DOI: 10.3791/69122-v

Dilan Gokyer¹, Natasha Salpeter¹, Lydia Hughes¹, Hoi Chang Lee¹, Meredith J Lohman¹, Tomiris Atazhanova¹, Anna Kleinhans², Joan K Riley^1,2, MaryEllen Pavone^1,2, Francesca E Duncan¹, Emily Zaniker-Gomez¹, Elnur Babayev^1,2

¹Department of Obstetrics and Gynecology, Feinberg School of Medicine,Northwestern University, ²Department of Obstetrics and Gynecology,Northwestern Medicine Center for Fertility and Reproductive Medicine

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该协议概述了从 IVF 程序中分离和处理卵泡液、体细胞和未成熟卵母细胞的标准化方法，从而实现高质量的分子和细胞分析。这些方法支持使用临床护理中通常丢弃的材料对生育、生殖衰老和卵巢功能障碍进行转化研究。

我们开发了可重复的方法，用于收集和分析体外受精副产物，以研究人类生育力、不孕症及生殖衰老。保持RNA完整性和细胞存活性是一项挑战，需要临床和研究团队之间快速协调的样本处理。首先，准备50毫升的锥形管，内含滤泡液。

以400 x g离心管，离心10分钟，温度为4摄氏度。将每管的上清液丢弃，留下细胞颗粒。向每个细胞沉淀加入4至5毫升DMEM/F-12，并通过温和移液法重新懸浮沉淀。

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