Encyclopedia Of Experiments

Prostate Cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Encyclopedia Of Experiments

Prostate Cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Sections

Assays and techniques

Animal model studies

Assays and techniques

Animal model studies

Assays and techniques

Peptide Purification

An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates

Protein Extraction and Proteolysis

A Method to Obtain Proteins from Prostate Tumor Tissue Samples and their Enzymatic Digestion into Peptides

ADI-based Autophagy Induction in Prostate Cancer Cells

An Enzyme-based Technique to Measure Autophagic Response in Cells

Agarose Spin Down Assay

A Technique to Embed Organoids for Histological Analysis

Prostate Organoid Assay

A Matrix Gel Ring-based Ex Vivo Culture Technique to Study the Differentiation Capacity of Prostate Epithelial Cells

Protein Lysate Extraction

A Technique to Lyse Organoids to Collect Cellular Proteins

Whole-mount Immunofluorescence Imaging

A Fixing and Staining Technique to Evaluate Intact Organoids

Sampling Prostate Cancer Tissue for Biobanking

An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue

Functionalized Wire-based Target Cell Isolation

An Ex Vivo Technique to Isolate Cancer Cells from Spiked Peripheral Blood

Single Cell Sampling Using Micromanipulation

A Technique to Isolate Single Cells from Cancer Cell Suspension

Single Cell Sampling Using Laser Capture Microdissection

A Technique to Harvest Target Cells from a Heterogeneous Cell Population

Preparing Organoids from Tumors

A 3D In Vitro Tumor Model

Generating Chemoresistant Prostate Cancer Cells

A Procedure for Obtaining Drug-resistant Cancer Cells In Vitro

Functional Chemoresistance Characterization

A Method to Evaluate Drug Resistance in Cancer Cells Using Clonogenic Assay

Phosphopeptide Enrichment

An Antibody-based Immunoprecipitation Technique to Separate Specific Phosphorylated Peptides from Complex Peptide Mixtures

Titanium Dioxide-based Enrichment of Phosphopeptides

A Method for the Selective Isolation of Phosphopeptides from Peptide Library

Animal model studies

Modeling Prostate Cancer in Genetically-engineered Mouse Models

A CRISPR/Cas9-mediated Localized Gene Editing Technique in Mouse Anterior Prostate Lobe Cells

Intra-prostatic Injection of Cancer Cells

A Technique to Deliver Cancer Cells for Establishing Orthotopic Prostate Cancer Mouse Model

Renal Capsule Tumor Xenografting

A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis

Extracting Prostate from Mouse Urogenital System

A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse

Individual Lobe Extraction from Prostate

A Technique to Harvest Individual Prostate Lobes from Mouse Prostate