Southern University of Science and Technology 9 articles published in JoVE Engineering Measuring the Mechanical Properties of Glass Fiber Reinforcement Polymer Composite Laminates Obtained by Different Fabrication Processes Jin Lai1, Xiaoyu Zhang1, Xin Zhang1 1Department of Mechanics and Aerospace Engineering, Southern University of Science and Technology This paper describes a fabrication process for fiber-reinforced polymer matrix composite laminates obtained using the wet hand lay-up/vacuum bag method. Environment Resource Recycling of Red Soil to Synthesize Fe2O3/FAU-type Zeolite Composite Material for Heavy Metal Removal Zheting Chu1, Jiaxin Liang1, Dazhong Yang1, Jing Li1, Hong Chen1 1State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Shenzhen Key Laboratory of Interfacial Science and Engineering, Southern University of Science and Technology This article presents a novel and convenient route to synthesize Fe2O3/faujasite (FAU)-type zeolite composite material from red soil. The detailed synthesis parameters have been finely tuned. The obtained composite material can be used for efficient heavy metal-contaminated water remediation, indicating its potential applications in environmental engineering. Biochemistry Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates Siyu Guo1, Zhao Liu1, Yongshuai Yang1, Jun Chen1, Chun Loong Ho1 1Department of Biomedical Engineering, Southern University of Science and Technology Here, we describe the use of inducer gradient plates to evaluate bacterial swarming motility while simultaneously obtaining multiple concentration responses. Bioengineering In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy Wanjie Wu1, Xuesong Li1, Jianan Y. Qu1,2,3,4, Sicong He5 1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice. Immunology and Infection Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity Xiaoyan Chang*1, Keru Zhou*1, Yanguang Liu1,2, Lian Duan3, Lichun Zhang2, Guoliang Zhang3, Haikun Wang4, Guiqin Wang1 1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity. Neuroscience WheelCon: A Wheel Control-Based Gaming Platform for Studying Human Sensorimotor Control Quanying Liu1,2,3, Yorie Nakahira2, Zhichao Liang1, Ahkeel Mohideen2, Adam Dai2, Sung Hoon Choi2, Angelina Pan2, Dimitar M. Ho2, John C. Doyle2 1Department of Biomedical Engineering, Southern University of Science and Technology, 2Division of Engineering and Applied Science, California Institute of Technology, 3Neuroscience Center, Huntington Medical Research Institutes WheelCon is a novel, free and open-source platform to design video games that noninvasively simulates mountain biking down a steep, twisting, bumpy trail. It contains components presenting in human sensorimotor control (delay, quantization, noise, disturbance, and multiple feedback loops) and allows researchers to study the layered architecture in sensorimotor control. Chemistry Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations HuiHui Hu1, DeBen Lu1, Kun Peng Dou1, Xing-Qiang Shi2 1College of Information Science and Engineering, Ocean University of China, 2Department of Physics, Southern University of Science and Technology Calculations performed by the Vienna Ab initio Simulation Package can be used to identify the intrinsic electronic properties of nanoscale materials and predict the potential water-splitting photocatalysts. Cancer Research Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography Yongyang Huang1, Jinyun Zou1, Mudabbir Badar1, Junchao Liu1, Wentao Shi5, Shunqiang Wang2, Qiongyu Guo3, Xiaofang Wang1, Sarah Kessel4, Leo Li-Ying Chan4, Peter Li4, Yaling Liu2,5, Jean Qiu4, Chao Zhou1,5,6 1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated. Biology Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM) Yilin Wang1, Pakorn Kanchanawong2,3 1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore We present a protocol for the application of interferometric PhotoActivated Localization Microscopy (iPALM), a 3-dimensional single-molecule localization super resolution microscopy method, to the imaging of the actin cytoskeleton in adherent mammalian cells. This approach allows light-based visualization of nanoscale structural features that would otherwise remain unresolved by conventional diffraction-limited optical microscopy.
