Vienna Biocenter (VBC) 2 articles published in JoVE Developmental Biology Protocol for Human Blastoids Modeling Blastocyst Development and Implantation Harunobu Kagawa*1, Alok Javali*1, Heidar Heidari Khoei*1, Theresa Maria Sommer1,3, Giovanni Sestini1,3, Maria Novatchkova2, Yvonne Scholte op Reimer1, Nicolas Rivron1 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 2Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells. Genetics Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes Wilson Pak-Kin Lou*1,2, Liqun Wang*3, Cheng Long1, Lei Liu3, Ji-Feng Fei3 1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.
