University of Exeter Medical School, Hatherly Laboratories 1 article published in JoVE Biochemistry Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting Sunday Solomon Josiah1, Nur Farah Meor Azlan1, Asami Oguro-Ando1, Jinwei Zhang1 1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories The present protocol highlights the application of western blotting technique to study the functions and activities of neuronal K-Cl co-transporter KCC2. The protocol describes the investigation of KCC2 phosphorylation at kinase regulatory sites Thr906/1007 via western blotting. Also, additional methods to confirm KCC2 activity are briefly highlighted in this text.
