Universidad de Guanajuato 1 article published in JoVE Neuroscience Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin Valeria Piazza1, Milvia Alata1, Victor H. Hernandez2, José R. Eguibar3,4, Carmen Cortes3 1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering, Universidad de Guanajuato, 3Institute of Physiology, Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional, Benemérita Universidad Autónoma de Puebla In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.
