University of California, Berkeley and University of California 1 article published in JoVE Bioengineering Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements Andre Lai*1, Rachel Rex*2, Kristen L. Cotner1, Alan Dong3, Michael Lustig1,3, Lydia L. Sohn1,2 1Graduate Program in Bioengineering, University of California, Berkeley and University of California, San Francisco, 2Department of Mechanical Engineering, University of California, Berkeley, 3Department of Electrical Engineering and Computer Sciences, University of California, Berkeley Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.
