Akadeum Life Sciences 1 article published in JoVE Immunology and Infection Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles Tiffany Snow1, Jon Roussey1, Casey Wegner1, Brandon McNaughton2 1Research and Development, Akadeum Life Sciences, 2Akadeum Life Sciences The objective of this study is to demonstrate the feasibility of flotation-based separation to isolate, activate, and expand primary human T cells.