JOVE JOURNAL

Biochemistry

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Journal (Biochemistry)

Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed E. coli Protein

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

James Sandy, Halina Mikolajek², Amy J. Thompson, Juan Sanchez-Weatherby, Michael A Hough²

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

In Situ X-ray Crystallographic Data Collection Using the Beamline at Diamond Light Source

James Sandy, Halina Mikolajek², Amy J. Thompson, Juan Sanchez-Weatherby, Michael A Hough²

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar, Danielle Dixon, Andrew M. Lipchik, Joshua J. Gruber

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar, Danielle Dixon, Andrew M. Lipchik, Joshua J. Gruber

Journal (Biochemistry)

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Myndert Claasen, Zornitsa Kofinova, Matteo Contino, Weston B. Struwe⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Measuring Biomolecular Interactions with Weaker Affinities Using Mass Photometry and Microfluidics

Myndert Claasen, Zornitsa Kofinova, Matteo Contino, Weston B. Struwe⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low-Affinity Protein-Protein Interactions

Myndert Claasen, Zornitsa Kofinova, Matteo Contino, Weston B. Struwe⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Yeast Cell Preparation for FRET Assay for Hyperosmotic Stress Analysis

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

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