Question 1

Why is DNA synthesized differently on the leading and lagging strands?

Accepted Answer

DNA polymerase can only synthesize DNA in the 5' to 3' direction. On the leading strand, this allows continuous synthesis in the direction of replication fork opening. On the lagging strand, synthesis must occur in the opposite direction, making continuous synthesis impossible. Therefore, lagging strand synthesis proceeds discontinuously in short fragments.

Question 2

What are Okazaki fragments and why are they formed?

Accepted Answer

Okazaki fragments are short DNA segments synthesized on the lagging strand. They form because DNA polymerase cannot synthesize in the 3' to 5' direction. Instead, primase repeatedly synthesizes RNA primers, and DNA polymerase fills between them, creating discontinuous fragments that are later joined together by DNA ligase.

Question 3

How does DNA primase contribute to lagging strand synthesis?

Accepted Answer

DNA primase synthesizes multiple RNA primers on the lagging strand as DNA unwinds at the replication fork. These primers provide the 3'-OH groups that DNA polymerase requires to begin synthesis. Primase works repeatedly along the lagging strand, enabling the discontinuous synthesis of multiple Okazaki fragments.

Question 4

What enzymes are involved in completing lagging strand synthesis?

Accepted Answer

After DNA polymerase synthesizes Okazaki fragments, RNase H removes the RNA primers between fragments. DNA polymerase I then fills the gaps left by primer removal. Finally, DNA ligase seals the nicks between fragments by joining the 3' end of one fragment to the 5' end of the next, creating a continuous strand.

Question 5

How do Okazaki fragment lengths differ between prokaryotes and eukaryotes?

Accepted Answer

Prokaryotic Okazaki fragments average 1000 to 2000 nucleotides in length, while eukaryotic fragments are much shorter at 100 to 200 nucleotides. This difference reflects the distinct genome sizes and replication machinery of prokaryotes and eukaryotes, requiring different coordination strategies during lagging strand synthesis.

Question 6

Why does lagging strand synthesis require more enzymes than leading strand synthesis?

Accepted Answer

Leading strand synthesis requires only a single primer and DNA polymerase for continuous replication. Lagging strand synthesis requires multiple enzymes—primase, DNA polymerase I, RNase H, and DNA ligase—because it proceeds discontinuously in fragments that must be individually primed, filled, and joined together.

Question 7

What is the role of DNA ligase in completing lagging strand synthesis?

Accepted Answer

DNA ligase catalyzes the final step of lagging strand synthesis by joining adjacent Okazaki fragments. It forms phosphodiester bonds between the 3' end of one fragment and the 5' end of the next, converting the discontinuous lagging strand into a continuous DNA molecule.

Synthese des nachlaufenden Strangs

Browse More Videos