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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

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Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

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Biologie

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JoVE Journal Biologie

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

DOI:

10.3791/200660-v

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01:43 min

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September 15, 2023

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Elena Levi-D’Ancona², Vaibhav Sidarala, Scott A. Soleimanpour^3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Tags

Single-cell Dissociation Murine Islets Flow Cytometric Analysis Î²-cell Mitophagy Islet Culture High-fat Diet Valinomycin Trypsin Cell Staining Flow Cytometry

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