Isolierung von Myofibroblasten aus Maus und Mensch Ösophagus

Overview

This protocol outlines the isolation of primary myofibroblasts from murine and human esophagus. The cultured cells exhibit a myofibroblast phenotype, characterized by spindle-shaped morphology and specific marker expression.

Key Study Components

Area of Science

• Cell biology
• Neuroscience
• Tissue engineering

Background

• Myofibroblasts play a crucial role in tissue repair and fibrosis.
• Understanding epithelial-stromal interactions is essential for various biological studies.
• Primary cultures provide a relevant model for studying these interactions.
• Isolation of myofibroblasts can enhance research in esophageal diseases.

Purpose of Study

• To develop a reliable method for isolating primary myofibroblasts.
• To characterize the isolated cells for functional studies.
• To facilitate research on epithelial-stromal interactions in the esophagus.

Methods Used

• Harvesting the esophagus from donor neonate mice.
• Separating mucosa from muscularis propria.
• Mincing and washing tissue in HBSS.
• Digesting tissue with collagenase to release mesenchymal cells.

Main Results

• Successful isolation of myofibroblasts with spindle-shaped morphology.
• Expression of α-SMA and vimentin confirmed myofibroblast phenotype.
• Absence of epithelial, hematopoietic, and endothelial markers in cultured cells.
• Cells are suitable for functional studies of epithelial-stromal interactions.

Conclusions

• The protocol effectively isolates primary myofibroblasts from esophageal tissue.
• Characterized cells can be utilized in further research.
• This method contributes to understanding the role of myofibroblasts in esophageal biology.

Frequently Asked Questions