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Isolieren von Stammzellen des Darms von Adult<em> Drosophila</em> Mitteldärmen durch FACS, um Stammzellverhalten Studie während des Alterns
Isolating Intestinal Stem Cells from Adult <em>Drosophila</em> Midguts by FACS to Study Stem Cell Behavior During Aging
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Isolieren von Stammzellen des Darms von Adult<em> Drosophila</em> Mitteldärmen durch FACS, um Stammzellverhalten Studie während des Alterns

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20,420 Views

10:57 min

December 16, 2014

DOI:

10:57 min
December 16, 2014

20380 Views
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Summary

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Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

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