Journal
/
Immunologie und Infektion
/
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

DOI:
10.3791/60397-v

•

09:43 min

•

December 07, 2019

•

, , , , , , ,
1Infections in Hematology and Oncology,Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology,Jena University Hospital, 3Institute for Transfusion Medicine,Jena University Hospital

Kapitel

  • 00:04Titel
  • 00:53Preparation of Aspergillus fumigatus Conidia
  • 02:26Preparation of Human Primary Leukocytes
  • 03:22Phagocytosis Assay
  • 04:01Antibody Staining
  • 05:58Flow Cytometry
  • 07:24Results: Measuring Phagocytosis
  • 08:58Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.

Tags

PhagocytosisAspergillus FumigatusConidiaHuman LeukocytesFlow CytometryCell MarkersNeutrophilsMonocytesMucoralesRed Blood CellsFITC LabelingRPMI MediumBuffy CoatEL BufferCentrifugation

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry

Read Article