Intracranial Pressure Monitoring In Nontraumatic Intraventricular Hemorrhage Rodent Model

Catherine Peterson; Cameron Hawk; Chloe H. Puglisi; Ben Waldau

doi:10.3791/63309

Intrakranielle Drucküberwachung im nichttraumatischen intraventrikulären Blutungsmodell

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Neuroscience

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JoVE Journal Neuroscience

Intracranial Pressure Monitoring In Nontraumatic Intraventricular Hemorrhage Rodent Model

Intrakranielle Drucküberwachung im nichttraumatischen intraventrikulären Blutungsmodell

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08:18 min

February 8, 2022

DOI: 10.3791/63309-v

Catherine Peterson¹, Cameron Hawk¹, Chloe H. Puglisi¹, Ben Waldau¹

¹Department of Neurological Surgery,UC Davis Medical Center

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study investigates a novel technique for monitoring intracranial pressure, mean arterial pressure, and cerebral perfusion pressure in a rat model of non-traumatic intraventricular hemorrhage (IVH). The method is designed to facilitate accurate and reliable measurements, which are crucial for evaluating the effects of IVH on cognitive functions in future research.

Key Study Components

Area of Science

Neuroscience
Pressure monitoring
Rodent models

Background

IVH can lead to increased intracranial pressure, necessitating monitoring techniques.
Existing methods in rodents are scarce in the literature.
Accurate measurements are critical for future cognitive studies related to IVH.

Purpose of Study

To establish a reliable methodology for measuring intracranial pressure in rat models.
To examine the impact of increased pressure on memory dysfunction following IVH.

Methods Used

The study employs a rat model to assess neurovascular parameters post-IVH.
Intracranial pressure was monitored using a fiber-optic sensor inserted into the cortex.
Mean arterial pressure was obtained via a sensor in the femoral artery.
Multiple surgical and monitoring steps were outlined for precise execution.

Main Results

Intracranial pressure increased significantly during IVH injections, with higher levels observed in IVH versus control groups.
Post-injection, the intracranial pressure normalized within five minutes.
Monitoring procedures established robust data for future cognitive evaluations.

Conclusions

This study demonstrates the feasibility of accurate ICP monitoring in a rodent model for studying IVH effects.
The methodology enables further exploration of cognitive dysfunctions related to increased intracranial pressure.

Frequently Asked Questions

What is the significance of measuring intracranial pressure in rodent models?

Measuring intracranial pressure is crucial for understanding the physiological responses in rodent models of brain injury, which can provide insights into similar conditions in humans.

How is the intracranial pressure measured in this study?

Intracranial pressure is measured using a fiber-optic sensor inserted into the rat's cortex, providing real-time data during experiments.

What challenges might researchers face when using this technique?

Challenges may include the small size of fiber-optic sensors and the microsurgical skills required for femoral artery dissection.

Why is it important to monitor both ICP and MAP?

Monitoring both intracranial pressure and mean arterial pressure allows researchers to assess cerebral perfusion pressure accurately, which is vital for understanding brain health and injury effects.

Can this method be applied to other brain injury models?

Yes, the methodology can be adapted for various models of brain injury to study different pathophysiological processes.

What potential insights could be gained from future studies using this model?

Future studies could elucidate mechanisms underlying cognitive dysfunctions associated with increased ICP following IVH, potentially leading to therapeutic advancements.

Die Überwachung des intrakraniellen Drucks in Nagetiermodellen nichttraumatischer intraventrikulärer Blutungen ist in der aktuellen Literatur nicht üblich. Hier demonstrieren wir eine Technik zur Messung des intrakraniellen Drucks, des mittleren arteriellen Drucks und des zerebralen Perfusionsdrucks während intraventrikulärer Blutungen in einem Rattentiermodell.

Dieses Protokoll beschreibt eine Methode zur Messung des intrakraniellen Drucks, des mittleren arteriellen Drucks und des zerebralen Perfusionsdrucks nach nicht-traumatischen intraventrikulären Blutungen bei Nagetieren. Intrakranielle und mittlere arterielle Drücke können genau und zuverlässig mit einem faseroptischen Sensor gemessen werden, der in den Kortex bzw. die Oberschenkelarterie der Ratte eingeführt wird. Die hier beschriebenen Techniken werden in das klinische Umfeld übertragen, wenn Patienten mit intraventrikulären Blutungen eine invasive intrakranielle Drucküberwachung benötigen.

Ziel dieser Studie war es, ein IVH-Tiermodell mit objektiver Überwachung von ICPs, MAPs und CCPs nach intraventrikulärer IVH zu etablieren, damit die Autoren dies in zukünftigen Experimenten weiter anwenden können, die sich auf die Auswirkungen von ICPs konzentrieren, die durch IVH induziert werden, auf nachfolgende Gedächtnisstörungen. Große Kämpfe können Präzision beinhalten, da faseroptische Sensoren klein sind. Die Sektion der Oberschenkelarterie könnte auch für einige eine Herausforderung darstellen, insbesondere für diejenigen, die nicht in mikrochirurgischen Fähigkeiten verwendet werden.

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