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Cancer Research

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JoVE Journal Cancer Research
Un approccio di segmentazione senza etichette per l'imaging intravitale del microambiente del tumore mammario
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Un approccio di segmentazione senza etichette per l'imaging intravitale del microambiente del tumore mammario

Article DOI: 10.3791/63413-v • 10:39 min • May 24th, 2022
May 24th, 2022 •
1, 1, 1,2, 1, 1,3
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Pathology, University of Wisconsin-Madison, 3Carbone Cancer Center, University of Wisconsin-Madison

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Il metodo di imaging intravitale qui descritto utilizza la seconda generazione armonica di collagene e la fluorescenza endogena dal cofattore metabolico NAD(P)H per segmentare in modo non invasivo un microambiente tumorale non etichettato in compartimenti tumorali, stromali e vascolari per l'analisi approfondita delle immagini intravitali 4D.

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