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Bioengineering

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JoVE Journal Bioengineering
Test af in vitro - og in vivo-effektiviteten af mRNA-lipidnanopartikler formuleret ved mikrofluidisk blanding
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Test af in vitro - og in vivo-effektiviteten af mRNA-lipidnanopartikler formuleret ved mikrofluidisk blanding

Article DOI: 10.3791/64810-v • 08:55 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1, 1, 1
1Department of Bioengineering, University of Pennsylvania

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Her præsenteres en protokol til formulering af lipidnanopartikler (LNP'er), der indkapsler mRNA-kodning af ildflueluciferase. Disse LNP'er blev testet for deres styrke in vitro i HepG2-celler og in vivo i C57BL/6-mus.

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Bioengineering udgave 191 In vitro-effektivitet in vivo-effektivitet LNP'er MRNA-last nukleasnedbrydning intracellulær levering ioniserbart lipid phospholipid kolesterol lipid-PEG-konjugat ildflueluciferase transfektionseffektivitet HepG2-celler bioluminescerende pladebaseret analyse C57BL/6-mus intravenøs injektion lateral halevene bioluminescensbilleddannelse i hele kroppen in vivo-billeddannelsessystem
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