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Neuroscience

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JoVE Journal Neuroscience
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Insights into the Techniques and Findings of Recent Advancements in Epilepsy Research (Video) | JoVE

Monitoring Epileptiform Events in Drosophila via Calcium Imaging

Article DOI: 10.3791/65825-v • 01:35 min • October 13th, 2023
October 13th, 2023 •
1, 2, 2, 2, 3,4, 1
1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

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Imagem de cálcio ex vivo Modelo de Drosophila Epilepsia Distúrbio neurológico Convulsões recorrentes Origem genética Tecnologia de imagem Indicadores de cálcio codificados geneticamente GCaMP6 Resolução em todo o cérebro Resolução de célula única Genética molecular Ensaios comportamentais Protocolo Drosophila adulta Atividades epileptiformes Microscópio confocal Atividade neural Ensaio de comportamento semelhante a convulsões sensível a Bang Moscas knockdown do gene Cac Atividades anormais de cálcio Triagem gênica patogênica
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