The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments on the plate reader utilize a standard curve to determine the experimental values. This curve uses samples of known concentration to generate a line of best fit or standard curve. Experimental values are then extrapolated to the curve or are calculated using the equation from the linear regression. Besides standards and samples being run on the multiwall plate, the blank along with positive and negative controls are also used in the assay to ensure it is working correctly. Multiplate readers are used to quantify protein, gene expression and various metabolic processes such as reactive oxygen species and calcium flux.…
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Embed code vs URL
An embed code allows you to place a JoVE video directly inside of your learning or training environment. Most learning management systems such as Canvas, Blackboard, and Moodle require an embed code for JoVE content to stream.
A URL allows you to place a link in programs such as PowerPoint or Microsoft Word which takes a user to a separate page where they can watch a JoVE video.
Most of the time, you will want to use the embed code. However, there are some environments that don’t support embed codes such as Google Classrooms and MS Teams. In those instances, you can use a URL to still provide access to JoVE videos.
What is an Embed Code?
Basically, it’s a snippet of code that you put on your website that displays as a video. An embed code pulls the video from the original source, allowing you to display a video without having to host the file on your website.
How to embed a video
STEP 1: COPY YOUR EMBED CODE
To generate the embed code, simply click the Copy button above. You can customize it by choosing a specific language for the video, or setting exact dimensions for the video player.
STEP 2: PASTE THE EMBED CODE INTO YOUR ENVIRONMENT
Embed codes can be used in most learning management systems (Canvas, Blackboard, Moodle…), organizational websites, and a host of other tools. The specifics of how to add in an embed code will depend on your environment.
Generally, it involves accessing a text editor, entering the HTML mode on the editor, and pasting in the embed code. Get more details on embedding into specific environments here.