JoVE Science Education

Immunology

(12 Videos)

Showing 1 - 12 in 12 videos.

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

Science Education (Immunology)

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

Science Education (Immunology)

ELISA Assays: Indirect, Sandwich, and Competitive

Science Education (Immunology)

ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes

Science Education (Immunology)

Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy

Science Education (Immunology)

Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas

Science Education (Immunology)

Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections

Science Education (Immunology)

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

Science Education (Immunology)

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

Science Education (Immunology)

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

Science Education (Immunology)

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

Science Education (Immunology)

Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability

Science Education (Immunology)