Zhejiang Chinese Medical University 6 articles published in JoVE Medicine Development and Evaluation of a Rat Model of Full-Thickness Cartilage Defects Haiyan Zhang*1, Ronghua Bao*2, Jiaan Xu3, Yanzhi Ge4, Zuxiang Chen1, Mengqiang Fan1, Guangping Yu5, Li Zhou1, Le Guo3, Letian Shan1, Hangxing Bao1 1The First Affiliated Hospital, Zhejiang Chinese Medical University, 2Fuyang Orthopaedics and Traumatology Affiliated Hospital, Zhejiang Chinese Medical University, 3College of Pharmaceutical Science, Zhejiang Chinese Medical University, 4Affiliated Zhejiang Hospital, Zhejiang University School of Medicine, 5Xianju County Hospital of Chinese Medicine This protocol establishes a full-thickness cartilage defects (FTCD) model by drilling holes in the femoral trochlear groove of rats and measuring the subsequent pain behavior and histopathological changes. Biochemistry Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles Xinghui Song1, Hao Shen2, Yanwei Li3, Yueting Xing3, Jiajia Wang3, Chun Guo3, Yingying Huang3, Jin Chen2 1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters. Medicine Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method Xiaoli Jin*1, Yang Li*2, Xuanwei Chen1, Jin Chen1, Jian Xu1 1School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 2School of Basic Medical Sciences, Fudan University Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method. Medicine Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats Jiaan Xu1,2, Li Yan1,2, Bo Yan1,2, Li Zhou1, Peijian Tong1, Letian Shan1 1The First Affiliated Hospital, Zhejiang Chinese Medical University, 2College of Pharmaceutical Science, Zhejiang Chinese Medical University This study describes the method of intra-articular injection of mono-iodoacetate in rats and discusses the resulting pain-related behaviors and histopathological changes, which provide references for future applications. Behavior Chronic Post-Ischemia Pain Model for Complex Regional Pain Syndrome Type-I in Rats Qimiao Hu*1, Xiaoli Zheng*1, Ruixiang Chen1, Boyu Liu1, Yan Tai2, Xiaomei Shao1, Jianqiao Fang1, Boyi Liu1 1Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, 2Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University Provided here is a protocol that details steps to establish an animal model of chronic post-ischemia pain (CPIP). This is a well-recognized model mimicking human complex regional pain syndrome type-I. Mechanical and thermal hypersensitivities are further evaluated, as well as capsaicin-induced nocifensive behaviors observed in the CPIP rat model. Medicine Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer's Disease and Mild Cognitive Impairment Mei Wang1, Zhengluan Liao2, Dewang Mao3, Qi Zhang1, Yumei Li3, Enyan Yu2, Zhongxiang Ding3 1Zhejiang Chinese Medical University, 2 Based on resting-state functional magnetic resonance imaging with Granger causality analysis, we investigated the alterations in the directed functional connectivity between the posterior cingulate cortex and whole brain in patients with Alzheimer's Disease (AD), patients with Mild Cognitive Impairment (MCI), and healthy controls.
