Ningbo University 9 articles published in JoVE Medicine Non-Intubated Video-Assisted Thoracoscopic Surgery Gao Bin*1,2, Luo Wenjun*3, Gui Yu2, Xu Zhipeng2, Zhu Binbin2, Yu Lina1 1Department of Anesthesia, Second Affiliated Hospital of Zhejiang University School of Medicine, 2Department of Anesthesia, The First Affiliated Hospital of Ningbo University, 3Health Science Center, Ningbo University Here, we present a non-intubated protocol for performing video-assisted thoracoscopic surgery with preserved autonomic breathing. Behavior Comparison of Kinetic Characteristics of Footwork during Stroke in Table Tennis: Cross-Step and Chasse Step Huiyu Zhou1,2, Yuqi He1, Xiaoyi Yang1, Feng Ren1, Ukadike C. Ugbolue2, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2School of Health and Life Sciences, University of the West of Scotland This study presents a protocol to investigate the ground reaction force characteristics between cross-step and chasse step during stroke in table tennis. Medicine Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model Yuan Yuan1, Meng-hua Chen1, Jing Huang2, Yuan Tian2, Ke Qin3, Zhang Yuan1, Wen-yan Wang1, Zhi-jiang Wu1, Xin-yue Tian1, Yubin Zhang4 1Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, 2Department of hepatobiliary surgery, The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University, 3Department of transplantation, The Second Affiliated Hospital of Guangxi Medical University, 4Digital Technology and Engineering college, Ningbo University of Finance & Economics This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation. Behavior Lower-Limb Biomechanical Characteristics Associated with Unplanned Gait Termination Under Different Walking Speeds Huiyu Zhou1,2, Xuanzhen Cen1, Yang Song3, Ukadike C. Ugbolue2, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2School of Health and Life Sciences, University of the West of Scotland, 3Faculty of Engineering, University of Szeged This study compared the biomechanical characteristics of the lower extremity during unplanned gait termination under different walking speeds. The lower-limb kinematic and kinetic data from fifteen subjects with normal and fast walking speeds were collected using a motion analysis system and plantar pressure platform. Behavior Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running Wenjing Quan1, Meizi Wang2, Gongju Liu2, Gusztáv Fekete3, Julien S. Baker4, Feng Ren1, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2Faculty of Engineering, University of Pannonia Veszeprem, 3Savaria Institute of Technology, Eötvös Loránd University, 4Department of Sport, and Physical Education, Hong Kong Baptist University This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively. Behavior Biomechanical Analysis Methods to Assess Professional Badminton Players' Lunge Performance Ping Huang1,2,3, Lin Fu1, Yan Zhang 3, Gusztáv Fekete4, Feng Ren1, Yaodong Gu1,2 1Faculty of Sports Science, Ningbo University, 2Research Academy of Grand Health Interdisciplinary, Ningbo University, 3Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, 4Savaria Institute of Technology, Eötvös Loránd University Here, we present a protocol to evaluate the differences in injury mechanisms between professional and amateur players when performing a badminton maximal right lunge movement by analyzing lower limb kinematics. Biochemistry A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis Huang Chunhui1,2, Xu Dilin1, Zhang Ke1, Shentu Jieyi2, Yan Sicheng1, Wu Dapeng3, Wang Qinwen1, Cui Wei1,2 1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, 3School of Materials Science and Chemical Engineering, Ningbo University This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis. Behavior Using Gold-standard Gait Analysis Methods to Assess Experience Effects on Lower-limb Mechanics During Moderate High-heeled Jogging and Running Yan Zhang 1,2,3, Meizi Wang1, Jan Awrejcewicz3, Gusztáv Fekete4, Feng Ren1, Yaodong Gu1,2 1Faculty of Sports Science, Ningbo University, 2Research Academy of Grand Health Interdisciplinary, Ningbo University, 3Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, 4Savaria Institute of Technology, Eötvös Loránd University This study investigated lower-limb kinematics and ground reaction force (GRF) during moderate high-heeled jogging and running. Subjects were divided into groups of experienced wearers and inexperienced wearers. A three-dimensional motion analysis system with a configured force platform captured lower-limb joint movements and GRF. Neuroscience Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture Lin Jiajia*1, Mak Shinghung*2, Zheng Jiacheng1, Wang Jialing1, Xu Dilin1, Hu Shengquan2, Zhang Zaijun3, Wang Qinwen1, Han Yifan2,4, Cui Wei1,2 1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.
